RNA

Part:BBa_K3009032:Design

Designed by: Alisa Zinina   Group: iGEM19_Freiburg   (2019-10-21)


MS2 stem loop structures can be fused to the untranslated 3`end of an mRNA sequence. The MS2 Capsid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3
    Illegal XbaI site found at 112
    Illegal PstI site found at 15
    Illegal PstI site found at 85
    Illegal PstI site found at 124
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 15
    Illegal PstI site found at 85
    Illegal PstI site found at 124
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3
    Illegal XbaI site found at 112
    Illegal PstI site found at 15
    Illegal PstI site found at 85
    Illegal PstI site found at 124
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3
    Illegal XbaI site found at 112
    Illegal PstI site found at 15
    Illegal PstI site found at 85
    Illegal PstI site found at 124
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

fused to untranslated 3' end (UTR(


Source

MS2 Bacteriophage

References